Performance evaluation of two immunoassays for 25-hydroxyvitamin D
نویسندگان
چکیده
منابع مشابه
Performance evaluation of two immunoassays for 25-hydroxyvitamin D
Although immunoassays in measuring 25-hydroxyvitamin D [25(OH)D] have been improved recently, relatively large differences are still seen between results of 25(OH)D measured by immunoassays and by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the present studies, we compared two immunoassays with LC-MS/MS for measuring 25(OH)D concentrations. Concentrations of 25-hydroxyvitamin ...
متن کاملSerum 25-hydroxyvitamin D immunoassays: recommendations for correct clinical interpretation.
assay on the Roche Modular analyzer. They used clinical samples that were almost free of 25hydroxyvitamin D2 (25-OH-D2) ( 10 nmol/L) as confirmed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) and found a good overall agreement between the results obtained with the Roche assay and those measured with the DiaSorin RIA and by LC-MS/MS. Although they acknowledged that the Roche assay...
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A total of 1628 human plasma samples from Cycle 1 of the Canadian Health Measures Survey were assayed for total 25-hydroxyvitamin D using the DiaSorin RIA method and the Diasorin “LIAISON 25 OH Vitamin D Total” method. Bland-Altman comparison showed an average bias of 4.8 ± 16.7 nmol/L (6.3%: P<0.001) with the LIAISON method giving higher values. The relationship was investigated using linear a...
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BACKGROUND Vitamin D is an endogenous hormone known to regulate calcium levels in the body and plays a role in cognitive performance. Studies have shown an association between vitamin D deficiency and cognitive impairment in older adults. Lebanon has a high 25-hydroxyvitamin D (25(OH)D) deficiency prevalence across all age groups. METHODS In this cross-sectional study, we explored the cogniti...
متن کاملDivergence in classification of 25-hydroxyvitamin D status with respect to immunoassays.
To the Editor: The major problem in measuring 25hydroxyvitamin D (25-OHD) is attributable to the molecule itself (1). Thus, an analytical method must be selected that will accurately estimate total circulating 25-OHD independent of the circulating concentrations of 25OHD2 and 25OHD3. Although HPLC remains the method of choice, many convergent data indicate that the 25-OHD RIA (DiaSorin Inc) sho...
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ژورنال
عنوان ژورنال: Journal of Clinical Biochemistry and Nutrition
سال: 2016
ISSN: 0912-0009,1880-5086
DOI: 10.3164/jcbn.15-61